|
KMID : 0811720070110000089
|
|
Korean Journal of Physiology & Pharmacology
2007 Volume.11 No. 0 p.89 ~ p.0
|
|
|
Endogenous TRPM4b-like Channel in Chinese Hamster Ovary Cells
|
|
Park Jae-Yong
Han Jae-Hee
Hong Seong-Geun
|
|
|
Abstract
|
|
|
Chinese hamster ovary (CHO) cells have been found to express endogenously Ca2+-activated cation channel. Biophysical and pharmacological properties of the channel have been studied using patch-clamp technique. The current-voltage relationship of the single channel current is almost linear in symmetrical 140 mM sodium aspartate. The slope conductance was 23.2±0.5 pS (n=7). The channel demonstrates almost no discrimination between Na+ and K+, having the similar permeability (PK/PNa=0.88±0.03, n=4). The channel is unlikely to be permeable to Ca2+ and Ba2+, which permeability ratios were 0.09±0.04 (n=7) and 0.08±0.05 (n=7) for Ca2+ and Ba2+, respectively. The activation of the channel occurred at micromolar range of cytosolic Ca2+ concentrations. Intracellular applied 100 μM ATP or ADP almost completely inhibited the channel activity. The channel was largely inhibited by intracellular spermine (1 mM). The biophysical and pharmacological properties of the observed channel are qualitatively similar to those of TRPM4b channel. Although the presence of TRPM4b-like channel was not confirmed at the DNA or protein level, CHO cells can be used as a model for studies related with TRPM4b channel function. This study was supported by the KRF-2004-005-E00008 and by MRC (R13-2005-012-01002-0) from KOSEF
Source: Korean Journal of Physiology & Pharmacology.2007 Oct;11(Suppl II):S83-S83
|
|
|
KEYWORD
|
|
|
TRPM4b, CHO cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|